Comparative Evaluation of Graphene Oxide, Zinc Oxide, and Zinc Sulfide Nanoparticles in Carbomer-HPMC for Enhanced Wound Healing
Authors
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Yousef Moradian Haftcheshmeh
Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0009-0009-0896-6284
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Tahereh Sadat Tabatabae
Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0009-0005-8129-944X
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Tayebeh Sadat Tabatabai
Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0000-0003-2077-3900
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Majid Salehi
Regenerative Medicine Research Center, Shahroud University of Medical Sciences, Shahroud, Iran; Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0000-0001-6283-8181
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Vahid Shirshahi
Department of Basic Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0000-0001-5181-7844
DOI:
https://doi.org/10.22100/ijhs.v12i1.1302Keywords:
Carbomer, HPMC, GO NPs, ZnS NPs, ZnO NPsAbstract
Background: Wounds are treated using various approaches, including hydrogel dressings and nanoparticles (NPs). Hydrogel dressings provide a cooling effect that promotes wound healing, while NPs such as graphene oxide (GO), zinc oxide (ZnO), and zinc sulfide (ZnS) enhance cellular activity and accelerate the healing process. This study aimed to investigate the effects of GO, ZnS, and ZnO NPs loaded with carbomer 980 and HPMC polymers on wound healing.
Methods: The mechanical and physical properties of the scaffolds were evaluated using FTIR, SEM, pH monitoring, degradation rate, water absorption, blood clotting index (BCI), and hemolysis tests. Biocompatibility and cell migration were assessed via MTT assay, DAPI staining, and scratch tests. In addition, full-thickness (15 mm × 15 mm) wounds were created in animal models, and wound healing was evaluated using Verhoeff-Van Gieson and hematoxylin-eosin staining.
Results: GO-based hydrogels exhibited superior biocompatibility, wettability, controlled degradation, enhanced cell viability, and accelerated cell migration compared to ZnS- and ZnO-based formulations when combined with carbomer 980 and HPMC polymers. Histological analysis revealed increased collagen and elastin synthesis, enhanced angiogenesis, and greater epidermal thickness.
Conclusions: The prepared hydrogels show promising potential for wound healing applications. Further clinical studies are warranted to validate their therapeutic efficacy.
References
Reinke J, Sorg HJEsr. Wound repair and regeneration. 2012;49(1):35-43. doi: 10.1159/000339613
Motley TA, Gilligan AM, Lange DL, Waycaster CR, Dickerson JEJJoF, Research A. Cost-effectiveness of clostridial collagenase ointment on wound closure in patients with diabetic foot ulcers: economic analysis of results from a multicenter, randomized, open-label trial. 2015;8:1-12. doi: 10.1186/s13047-015-0065-x
Boyce ST, Lalley ALJB, trauma. Tissue engineering of skin and regenerative medicine for wound care. 2018;6 doi: 10.1186/s41038-017-0103-y
Alavi A, Sibbald RG, Mayer D, et al. Diabetic foot ulcers: part II. Management. 2014;70(1):21. e1-21. e24. doi: 10.1016/j.jaad.2013.07.048
Fajriyah NN, Slamet S, Waznah U, et al. Topical nano hydrogel formulation of annona muricata extract enrich with zinc for improved therapy design and evaluation. AIP Publishing; 2023: doi: 10.1063/5.0120738
Jaworski Z, Spychaj T, Story A, Story GJRiCE. Carbomer microgels as model yield-stress fluids. 2022;38(7):881-919. doi: 10.1515/revce-2020-0016
Dahl T, Calderwood T, Bormeth A, Trimble K, Piepmeier EJJocr. Influence of physico-chemical properties of hydroxypropyl methylcellulose on naproxen release from sustained release matrix tablets. 1990;14(1):1-10. doi: 10.1016/0168-3659(90)90055-X
Siepmann J, Peppas NAJAddr. Modeling of drug release from delivery systems based on hydroxypropyl methylcellulose (HPMC). 2012;64:163-174. doi: 10.1016/j.addr.2012.09.028
Hajipour MJ, Fromm KM, Ashkarran AA, et al. Antibacterial properties of nanoparticles. 2012;30(10):499-511. doi: 10.1016/j.tibtech.2012.06.004
Kalpana V, Devi Rajeswari VJBc, applications. A review on green synthesis, biomedical applications, and toxicity studies of ZnO NPs. 2018;2018(1):3569758. doi: 10.1155/2018/3569758
Han B, Fang WH, Zhao S, Yang Z, Hoang BXJNN, Biology, Medicine. Zinc sulfide nanoparticles improve skin regeneration. 2020;29:102263. doi: 10.1016/j.nano.2020.102263
Kaushik M, Niranjan R, Thangam R, et al. Investigations on the antimicrobial activity and wound healing potential of ZnO nanoparticles. 2019;479:1169-1177. doi: 10.1016/j.apsusc.2019.02.189
Shirshahi V, Tabatabaei SN, Hatamie S, Saber RJJoP, Analysis B. Functionalized reduced graphene oxide as a lateral flow immuneassay label for one-step detection of Escherichia coli O157: H7. 2019;164:104-111. doi: 10.1016/j.jpba.2018.09.048
Saedi M, Shirshahi V, Mirzaii M, Nikbakht MJJoDS, Technology. Preparation of graphene oxide nanoparticles and their derivatives: Evaluation of their antimicrobial and anti-proliferative activity against 3T3 cell line. 2024;45(3):381-389. doi: 10.1080/01932691.2022.2151458
Shirshahi V, Saedi M, Nikbakht M, Mirzaii MJJoDDS, Technology. Unveiling the antimicrobial potential of oxidized graphene derivatives: promising materials for advanced wound dressings and antibacterial surfaces. 2023;88:104949. doi: 10.1016/j.jddst.2023.104949
Ma R, Wang Y, Qi H, et al. Nanocomposite sponges of sodium alginate/graphene oxide/polyvinyl alcohol as potential wound dressing: In vitro and in vivo evaluation. 2019;167:396-405. doi: 10.1016/j.compositesb.2019.03.006
Mahmoudi N, Eslahi N, Mehdipour A, et al. Temporary skin grafts based on hybrid graphene oxide-natural biopolymer nanofibers as effective wound healing substitutes: pre-clinical and pathological studies in animal models. 2017;28:1-13. doi: 10.1007/s10856-017-5874-y
Karimi F, Alizadeh M, Bitaraf FS, Shirshahi V. Enhancing electrical conductivity and mechanical properties of decellularized umbilical cord arteries using graphene coatings. Journal of Biomedical Materials Research Part B: Applied Biomaterials 2024;112(7):e35448. doi: 10.1002/jbm.b.35448
Agubata CO, Okereke C, Nzekwe IT, Onoja RI, Obitte NCJEJoPS. Development and evaluation of wound healing hydrogels based on a quinolone, hydroxypropyl methylcellulose and biodegradable microfibres. 2016;89:1-10. doi: 10.1016/j.ejps.2016.04.017
Prabu D, Majdalawieh AF, Abu-Yousef IA, et al. Preparation and characterization of gatifloxacin-loaded sodium alginate hydrogel membranes supplemented with hydroxypropyl methylcellulose and hydroxypropyl cellulose polymers for wound dressing. 2016;6(2):86. doi: 10.4103/2230-973X.177810
Ismail SH, Hamdy A, Ismail TA, Mahboub HH, Mahmoud WH, Daoush WMJC. Synthesis and characterization of antibacterial carbopol/ZnO hybrid nanoparticles gel. 2021;11(9):1092. doi: 10.3390/cryst11091092
Kaushik M, Niranjan R, Thangam R, et al. Investigations on the antimicrobial activity and wound healing potential of ZnO nanoparticles. Applied Surface Science 2019;479:1169-1177. doi: 10.1016/j.apsusc.2019.02.189
Cleetus CM, Alvarez Primo F, Fregoso G, et al. Alginate hydrogels with embedded ZnO nanoparticles for wound healing therapy. International journal of nanomedicine 2020:5097-5111. doi: 10.2147/IJN.S255937
Wang Z, Hu Y, Xue Y, et al. Log P determines licorice flavonoids release behaviors and classification from CARBOMER cross-linked hydrogel. 2022;14(7):1333. doi: 10.3390/pharmaceutics14071333
Wang Z, Xue Y, Zhu Z, et al. Quantitative structure-activity relationship of enhancers of licochalcone a and glabridin release and permeation enhancement from carbomer hydrogel. 2022;14(2):262. doi: 10.3390/pharmaceutics14020262
Zaltariov M-FJCCTCCT. FTIR investigation on crystallinity of hydroxypropyl methyl cellulose-based polymeric blends. 2021;55:981-988. doi: 10.35812/CelluloseChemTechnol.2021.55.84
Surekha G, Krishnaiah KV, Ravi N, Suvarna RP. FTIR, Raman and XRD analysis of graphene oxide films prepared by modified Hummers method. IOP Publishing; 2020:012012. doi: 10.1088/1742-6596/1495/1/012012
El-Belely EF, Farag MM, Said HA, et al. Green synthesis of zinc oxide nanoparticles (ZnO-NPs) using Arthrospira platensis (Class: Cyanophyceae) and evaluation of their biomedical activities. 2021;11(1):95. doi: 10.3390/nano11010095
Abdelghany A, Meikhail M, Oraby A, Aboelwafa MJPB. Experimental and DFT studies on the structural and optical properties of chitosan/polyvinyl pyrrolidone/ZnS nanocomposites. 2023;80(12):13279-13298. doi: 10.1007/s00289-023-04700-0
Gharagozlou M, Naghibi SJMRB. Sensitization of ZnO nanoparticle by vitamin B12: Investigation of microstructure, FTIR and optical properties. 2016;84:71-78. doi: 10.1016/j.materresbull.2016.07.029
Pouran HM, Llabjani V, Martin FL, Zhang HJEs, technology. Evaluation of ATR-FTIR spectroscopy with multivariate analysis to study the binding mechanisms of ZnO nanoparticles or Zn2+ to chelex-100 or metsorb. 2013;47(19):11115-11121. doi: 10.1021/es4017552
Praseptiangga D, Zahara HL, Widjanarko PI, Joni I, Panatarani C. Preparation and FTIR spectroscopic studies of SiO2-ZnO nanoparticles suspension for the development of carrageenan-based bio-nanocomposite film. AIP Publishing; 2020: doi: 10.1063/5.0003434
Srujana S, Bhagat DJNfEE. Chemical-based synthesis of ZnO nanoparticles and their applications in agriculture. 2022;7(1):269-275. doi: 10.1007/s41204-022-00224-6
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