Shahroud University of Medical siences Shahroud Journal of Medical Sciences 2423-6594 11 4 2025 11 05 Comparative Evaluation of Graphene Oxide, Zinc Oxide, and Zinc Sulfide Nanoparticles in Carbomer-HPMC for Enhanced Wound Healing 68 82 10.22100/ijhs.v11i4.1302 eng Yousef Moradian Haftcheshmeh Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.. yousofmoradian@gmail.com Tahereh Sadat Tabatabae Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.. tabatabae14021@gmail.com Tayebeh Sadat Tabatabai Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.. taybeh.sadat.tabatabaie1400@gmail.com Majid Salehi Regenerative Medicine Research Center, Shahroud University of Medical Sciences, Shahroud, Iran; Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.. salehi.m@shmu.ac.ir Vahid Shirshahi Department of Basic Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.. shirshahi@gmail.com 2025 07 13 2025 10 06 Background: Wounds are treated using various approaches, including hydrogel dressings and nanoparticles (NPs). Hydrogel dressings provide a cooling effect that promotes wound healing, while NPs such as graphene oxide (GO), zinc oxide (ZnO), and zinc sulfide (ZnS) enhance cellular activity and accelerate the healing process. This study aimed to investigate the effects of GO, ZnS, and ZnO NPs loaded with carbomer 980 and HPMC polymers on wound healing. Methods: The mechanical and physical properties of the scaffolds were evaluated using FTIR, SEM, pH monitoring, degradation rate, water absorption, blood clotting index (BCI), and hemolysis tests. Biocompatibility and cell migration were assessed via MTT assay, DAPI staining, and scratch tests. In addition, full-thickness (15 mm × 15 mm) wounds were created in animal models, and wound healing was evaluated using Verhoeff-Van Gieson and hematoxylin-eosin staining. Results: GO-based hydrogels exhibited superior biocompatibility, wettability, controlled degradation, enhanced cell viability, and accelerated cell migration compared to ZnS- and ZnO-based formulations when combined with carbomer 980 and HPMC polymers. Histological analysis revealed increased collagen and elastin synthesis, enhanced angiogenesis, and greater epidermal thickness. Conclusions: The prepared hydrogels show promising potential for wound healing applications. Further clinical studies are warranted to validate their therapeutic efficacy.