The Beneficial Effects of Exercise and Date Palm Pollen Supplement on Liver Histopathology in the Cadmium Chloride Toxicity Model
Authors
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Amir Delshad
Department of Sport Science, Faculty of Literature and Humanities, Qom University, Qom, Iran.
https://orcid.org/0000-0002-3244-1180
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Maryam Azimzadeh
Department of Medical Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran.
https://orcid.org/0000-0003-1044-4177
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Fereshteh Salimi
Department of Sport Science, Faculty of Literature and Humanities, Qom University, Qom, Iran.
https://orcid.org/0000-0002-4176-3894
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Mohsen Eslami Farsani
Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran;Anatomy Department, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
https://orcid.org/0000-0001-6197-8350
- Shima Ababzadeh Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran; Department of Tissue Engineering and Applied Cell Sciences Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
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Reihaneh Seyedebrahimi
Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran;Anatomy Department, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
https://orcid.org/0000-0003-2550-5550
DOI:
https://doi.org/10.22100/ijhs.v12i1.1290Keywords:
Cadmium chloride, Exercise, Date palm pollen, LiverAbstract
Background: Cadmium (Cd) is a highly toxic heavy metal that poses a significant health risk in vital organs such the liver. This study investigates the protective effects of aerobic exercise and hydroalcoholic extract of Date Palm Pollen (DPP) in rat Cd toxicity model.
Methods: Thirty-six male Wistar rats (230-250 g) divided to six experimental groups (n=6): control, exercise, Cd, Cd-exercise, Cd- exercise-200 DPP, and Cd-Exercise-400 DPP. Each group received a daily dose of 3mg/kg of Cd chloride via gavage. Then, DPP extract was administered intraperitoneally at doses of 200 and 400 mg/kg/day. The aerobic exercise protocol was involved the rats running on a treadmill at a speed of 27 meters/min, with an additional minute added to the training duration each day. The enzyme levels (AST, ALT, GGT) were measured and the histopathological analysis of liver tissue were performed after 5 weeks.
Results: Results demonstrated that treatment groups have the significant decrease of GGT and ALT serum levels compared with Cd group (GGT; P-value<0.001, ALT; P-value<0.05). However, there were no significant difference in AST serum level between different groups. Besides, the histopathological findings revealed the number of necrotic areas, sinusoids congestion, and inflammation in Cd group while these alternations were decreased in treatment groups compared with injured group.
Conclusions: The findings demonstrating that the combination of aerobic exercise and DPP extract effectively reduce inflammation and cellular damage in liver tissue. So, DPP extract with 200 mg/kg concentration along with regular exercise has hepatoprotective potential effect.
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