Shahroud University of Medical siences Shahroud Journal of Medical Sciences 2423-6594 12 1 2025 11 30 The Beneficial Effects of Exercise and Date Palm Pollen Supplement on Liver Histopathology in the Cadmium Chloride Toxicity Model 23 26 eng Amir Delshad Department of Sport Science, Faculty of Literature and Humanities, Qom University, Qom, Iran.. ah_delshad@yahoo.com Maryam Azimzadeh Department of Medical Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran.. maryam.azimzadeh91@gmail.com Fereshteh Salimi Department of Sport Science, Faculty of Literature and Humanities, Qom University, Qom, Iran.. fereshte.salimi13@gmail.com Mohsen Eslami Farsani Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran;Anatomy Department, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran. ahoramh.82@gmail.com Shima Ababzadeh Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran; Department of Tissue Engineering and Applied Cell Sciences Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.. shimaababzadeh@gmail.com Reihaneh Seyedebrahimi Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran;Anatomy Department, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran. reihaneh.s.ebrahimi@gmail.com 2025 06 30 2025 10 07 Background: Cadmium (Cd) is a highly toxic heavy metal that poses a significant health risk in vital organs such the liver. This study investigates the protective effects of aerobic exercise and hydroalcoholic extract of Date Palm Pollen (DPP) in rat Cd toxicity model. Methods: Thirty-six male Wistar rats (230-250 g) divided to six experimental groups (n=6): control, exercise, Cd, Cd-exercise, Cd- exercise-200 DPP, and Cd-Exercise-400 DPP. Each group received a daily dose of 3mg/kg of Cd chloride via gavage. Then, DPP extract was administered intraperitoneally at doses of 200 and 400 mg/kg/day. The aerobic exercise protocol was involved the rats running on a treadmill at a speed of 27 meters/min, with an additional minute added to the training duration each day. The enzyme levels (AST, ALT, GGT) were measured and the histopathological analysis of liver tissue were performed after 5 weeks. Results: Results demonstrated that treatment groups have the significant decrease of GGT and ALT serum levels compared with Cd group (GGT; P-value<0.001, ALT; P-value<0.05). However, there were no significant difference in AST serum level between different groups. Besides, the histopathological findings revealed the number of necrotic areas, sinusoids congestion, and inflammation in Cd group while these alternations were decreased in treatment groups compared with injured group. Conclusions: The findings demonstrating that the combination of aerobic exercise and DPP extract effectively reduce inflammation and cellular damage in liver tissue. So, DPP extract with 200 mg/kg concentration along with regular exercise has hepatoprotective potential effect.